Fig. 3

The increase of miR-182 in BMSCs-Exos was closely correlated to hypoxia induction. BD-BMSCs were cultured under normoxic or hypoxic conditions. A, B qRT-PCR was performed to detect the expression of miR-182 in A BD-BMSCs and B BD-BMSCs secreted exosomes. C Image of HIF-1α motif. D ChIP assay was conducted to examine the binding relationship between HIF-1α and miR-182 promoter. BD-BMSCs were transfected with lv-HIF-1α or lv-NC, respectively. E, F qRT-PCR was used to detect the expression of E HIF-1α and F miR-182. G The dual luciferase assay was employed to investigate the impact of HIF-1α on the transcriptional activity of miR-182