Fig. 5
From: MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression
EIF4G2 reverses the promotion of miR-877-5p upregulation on osteoblast differentiation. (A) RT-qPCR and Western blot to verify the successful co-transfection of miR-877-5p mimic and oe-EIF4G2; (B/C) alizarin red staining to detect the mineralization of MC3T3-E1 cells; D: ALP activity assay; (E–G) RT-qPCR detection of ALP, COLIA1, and OPN; the values are expressed as mean ± standard deviation; *P < 0.05 versus miR-877-5p mimic + oe-NC