Fig. 6

Inhibition of TP53INP1 alleviated the functions of down-regulated miR-26b-5p. A The expression levels of TP53INP1 of MG63/DDP and Saos2/DDP cells were detected by qRT-PCR after transfection. B Cell viability determined by CCK-8 assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with sh-TP53INP1 and miR-26b-5p inhibitor, **P < 0.01; ***P < 0.001. C, D. Cell proliferation detected by EdU assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with sh-TP53INP1 and miR-26b-5p inhibitor. E, F Cell apoptosis determined by flow cytometry assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with sh-TP53INP1 and miR-26b-5p inhibitor. G, H Western blotting assay analyses of expression levels of caspase-3, Bax, as well as Bcl-2 in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with sh-TP53INP1 and miR-26b-5p inhibitor, **P < 0.01; ***P < 0.001. The concentration of DDP is 2 μg/mL