Fig. 5

TP53INP1 was a target gene of miR-26b-5p. A Venn diagram of downstream genes of miR-26b-5p were predicted through three bioinformatic tools. B Biological pathways of 111 genes enrichment analyzed by FunRich. C Binding sites between miR-26b-5p and wild-type TP53INP1. D Dual-luciferase reporter assay demonstrated that miR-26b-5p bind with wild-type TP53INP1 3’UTR, **P < 0.01. E Biotin RNA pull-down assay results demonstrated that TP53INP1 was gathered in miR-26b-5p biotin probe compared with negative control, **P < 0.01. F mRNA expression levels of TP53INP1 after transfection, ***P < 0.001. G qRT-PCR analyses of TP53INP1 in osteoblast and osteosarcoma cell lines including two DDP-resistant cells