Fig. 4

Up-regulated miR-26b-5p reversed the effects of GAS5 on cellular functions of MG63/DDP and Saos2/DDP cells. A miR-26b-5p expression levels of MG63/DDP and Saos2/DDP were determined by qRT-PCR after transfection. B. Cell viability detected by CCK-8 assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with GAS5 and miR-26b-5p over-expression plasmids, **P < 0.01; ***P < 0.001. C, D. Cell proliferation detected by EdU assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with GAS5 and over-expressed miR-26b-5p plasmids. E, F Cell apoptosis determined by flow cytometry assay in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with GAS5 and up-regulated miR-26b-5p plasmids. G, H Western blotting assay analyses of expression levels of caspase-3, Bax, and Bcl-2 in MG63/DDP and Saos2/DDP cells treated with DDP and co-transfected with GAS5 and miR-26b-5p mimic, *P < 0.05; **P < 0.01; ***P < 0.001. The concentration of DDP is 2 μg/mL