Fig. 3

GAS5 acted as a miR-26b-5p sponge in MG63/DDP and Saos2/DDP cells. A Venn diagram of miRNAs that bind with GAS5 were predicted through three bioinformatic tools. B mRNA level of 6 miRNAs in hFOB1.19 and osteosarcoma cells, **P < 0.01. C Interactions between miR-26b-5p and wild-type GAS5. D Dual-luciferase reporter assay demonstrated that miR-26b-5p bind with wild-type GAS5 3’UTR, **P < 0.01. E Biotin RNA pull-down assay results demonstrated that GAS5 was gathered in miR-26b-5p biotin probe compared to negative control, **P < 0.01. F mRNA expression levels of miR-26b-5p after down-regulation of GAS5 or over-expression of GAS5, **P < 0.01; ***P < 0.001. G. qRT-PCR analyses of miR-26b-5p in osteoblast and osteosarcoma cell lines including two DDP-resistant cells