Fig. 2

Suppression of GAS5 inhibited cell viability and facilitated apoptosis of DDP-resistant osteosarcoma cells. A qRT-PCR analyses of GAS5 expression in MG63/DDP and Saos2/DDP cells after lentiviral transfection. B Cell viability evaluated by CCK-8 assay in MG63/DDP and Saos2/DDP cells treated with DDP and transfected with GAS5, **P < 0.01; ***P < 0.001. C, D. Cell proliferation detected by EdU assay in MG63/DDP and Saos2/DDP cells treated with DDP and transfected with GAS5. E, F. Cell apoptosis determined by flow cytometry assay in MG63/DDP and Saos2/DDP cells treated with DDP and transfected with GAS5. G, H. Western blotting assay analyses of expression levels of cleaved-caspase-3, Bax, as well as Bcl-2 in MG63/DDP and Saos2/DDP cells treated with DDP and transfected with GAS5, *P < 0.05; **P < 0.01. The concentration of DDP is 2 μg/mL