Fig. 1

GAS5 was reduced in DDP-resistant osteosarcoma cells. A qRT-PCR analyses of GAS5 expression in osteosarcoma tissues and adjacent controls. B qRT-PCR analyses of GAS5 levels in osteosarcoma tissues from DDP-resistant and DDP-sensitive patients. C The secondary structures of GAS5 analyzed by RNAfold web server. D–F. Cellular functions measured by CCK-8, EdU staining and flow cytometry assays. G GAS5 was mainly localized in the cytoplasm of osteosarcoma cells analyzed by FISH. H mRNA level of GAS5 in osteoblast and osteosarcoma cell lines including two DDP-resistant cells