Fig. 6

Changes of serum bone turnover markers in mice. A–D The serum levels of CTX-1, TRACP5b, PINP and ALP in mice of each groups at 16, 24 and 32 weeks were evaluated by ELISA. E–H The mRNA expression levels of Runx2, Osx, OPG, and RANKL in tibiae of each groups at 16, 24 and 32 weeks were detected by RT-PCR. *P < 0.05, **P < 0.01 versus WT group; ^△P < 0.05 versus HFD group at 24 weeks