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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Identification of sulfur metabolism-related gene signature in osteoarthritis and TM9SF2’s sustenance effect on M2 macrophages' phagocytic activity

Fig. 6

The effect of TM9SF2 on phagocytosis of macrophages M2. A, B Knocking down TM9SF2 in THP-1-derived macrophages M2 at mRNA (A) and protein (B) levels. C The downregulation of macrophage phagocytosis on PE-stained IgG-coated latex beads by knocking down TM9SF2. D Downregulation of macrophage phagocytosis on PKH26-stained apoptotic Jurkat cells following TM9SF2 knockdown. Flow cytometry and immunofluorescence results with statistical values obtained from three biological replicates in one technical replicate. The data are representative of three independent experiments. *P < 0.05, two-sided t-test. Red scale bar: 50 μm

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X