Fig. 4

Validation of the diagnostic six-gene signature in GSE12021 and GSE1919 datasets. A, B Accuracy of the diagnostic model for the six-gene signature in predicting osteoarthritis by ROC analysis in GSE12021 (A) and GSE1919 (B) datasets. C, D Boxplot shows the differential expression of the six genes between healthy and OA groups in the GSE12021 (C) and GSE1919 (D) datasets. E, F Detailed diagnostic information (healthy/OA) and expression patterns of candidate genes between high-risk score and low-risk score groups in the GSE12021 (E) and GSE1919 (F) datasets, thus validating the diagnostic performance of the six-gene signature. Upper layer: risk score for each sample; middle layer: OA status (red: OA, green: healthy); lower layer: heatmap shows the gene expression