Fig. 4

Circ_0020014 was verified as a miR-24-3p sponge. A Schematic illustration demonstrating complementary to the miR-24-3p seed sequence with circ_0020014. B RT-qPCR demonstrated the transfection efficiency of miR-24-3p mimic. C The relative luciferase activities were detected after co-transfection of WT-circ_0020014 or MUT-circ_0020014 and miR-24-3p mimic or miR-NC in CHON-001 cells. D RT-qPCR detection of the enrichment of circ_0020014 and miR-24-3p in the complex precipitated by the anti-Ago2 or anti-IgG antibody. E RT-qPCR evaluation of the abundance of circ_0020014 in the complex pulled down by the bio-miR-24-3p or bio-miR-NC probe. F Relative expression of miR-24-3p in articular cartilages from OA patients and normal controls. G Pearson’s correlation coefficient assessment of the correlation between circ_0020014 and miR-24-3p expression levels in articular cartilages from OA patients. H Relative expression of miR-24-3p in CHON-001 cells treated with different doses of IL-1β. **P < 0.01, ***P < 0.001, and ****P < 0.0001