Fig. 8

ITGB1 ameliorates chondrocyte damage through the cAMP pathway in C28/I2 cells (N = 3). A RT-qPCR was used to detect ITGB1 expression in normal chondrocytes of each group. B RT-qPCR was used to detect ITGB1 expression in OA model chondrocytes of each group. C ELISA detected the cAMP concentration in each group. D The levels of PKA and p-PKA were detected by western blot. E Cell Counting Kit-8 assay detected cell viability. F The levels of IL-1β, TNF-α, and IL-6 were detected by ELISA. G Apoptotic chondrocytes were quantified by flow cytometry analysis with fluorescence staining. ^**p < 0.01, ^***p < 0.001 versus Control; ^#p < 0.05, ^##p < 0.01 versus LPS+oe-NC; ^&p < 0.05, ^&&p < 0.01 versus LPS+oe-ITGB1