Fig. 5

BRD4 regulated the expression of WNT4. A The interaction between BRD4 and WNT4 was detected by ChIP. B–F BRD4 overexpression vector or vector was transfected into hBMSCs. B Detection for mRNA expression of BRD4 using qRT-PCR. C Western blot analysis was used to examine the protein expression of BRD4. D The binding of BRD4 to WNT4 promoter was detected using dual-luciferase reporter assay. E Detection for mRNA expression of WNT4 using qRT-PCR. F The protein expression of WNT4 was detected by Western blot. n = 3; **P < 0.01, ***P < 0.001