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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway

Fig. 3

Effect of shBRD4 on osteogenic differentiation of hBMSCs. hBMSCs were transfected with shNC or shBRD4. A Detection of BRD4 mRNA expreesion by qRT-PCR. B Detection of BRD4 protein expression using Western blot analysis. C The ALP activity was measured using ALP Assay Kit. D ARS was performed for assessing mineralized deposit. E The protein expression levels of OCN, RUNX2, BSP and ALP were determined using Western blot. n = 3; **P < 0.01, ***P < 0.001

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X