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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway

Fig. 2

BRD4 expression in osteogenic differentiation of hBMSCs. hBMSCs were induced with or without osteogenic differentiation. A The ALP activity was detected by ALP Assay Kit. B ARS was used to detect mineralized deposit. C The protein expression levels of OCN, RUNX2, BSP and ALP were determined using Western blot analysis. D Detection of BRD4 mRNA expression by qRT-PCR. E Detection of BRD4 protein expression by Western blot. n = 3; **P < 0.01, ***P < 0.001

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X