Fig. 5

Overexpression of SOX4 partially reverse the inhibitory effects of miR-3195 for OS progression. A-B Relative expression of SOX4 mRNA and protein after MG-63 cells co-transfected with miR-3195 mimics and SOX4 plasmid. C CCK-8 assays was performed to evaluate the cell viability after MG-63 cells co-transfected with miR-3195 mimics and SOX4 plasmid. D Colony formation ability was measured in MG-63 cells co-transfected with miR-3195 mimics and SOX4 plasmid. E The level of apoptosis rate in co-transfected with miR-3195 mimics and SOX4 plasmid was detected by flow cytometry. F Images of measurements of cell migration in vitro scratch assay with MG-63 cells co-transfected with miR-3195 mimics and SOX4 plasmid at 0 h and 24 h. Scale bars are 200 µm. G Transwell invasion assay was performed in MG-63 cells co-transfected with miR-3195 mimics and SOX4 plasmid. Scale bars are 100 µm Data are presented as mean ± SD of three independent experiments. *indicates P < 0.05, ***indicates P < 0.001