Fig. 4

SOX4 is a direct target of miR-3195. A The volcano plot screen differentially expressed mRNAs in GSE16088 database. B Venn diagram of predicted target genes using TargetScan database and differentially expressed genes in GSE16088. C Expression level of SOX4 mRNA in OS tissues and adjacent normal tissues. D Correlation analysis between SOX4 and miR-3195 expression levels in OS tissues was analysed by Pearson's correlation analysis. E The wild-type (WT) and mutant (mut) 3’-UTR (untranslated region) of SOX4 contained the complementary sequences of miR-3195. F Luciferase activity was measured in MG-63 cells co-transfected with miR-3195 mimics or mimics NC and wild type (wt) or mutant (mut) 3ʹUTR of SOX4. (G-H) Relative expression of SOX4 mRNA and protein after overexpressing miR-3195 were assessed by qRT-PCR and Western blot, respectively. Data are presented as mean ± SD of three independent experiments. ***indicates P < 0.001, ns indicates P > 0.05