Fig. 3
From: miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4
Overexpression of miR-3195 suppressed the migration and invasion capacity of OS cells. A After transfection with miR-3195 mimics, miR-NC, OS monolayers were scratched to induce horizontal migration of U2OS and MG-63 cells at 0 h and 24 h. Scale bars are 200 µm; B Transwell invasion assay was performed in U2OS and MG-63 cells transfected with miR-3195 mimics, miR-NC. At 24 h after plating, cells that had migrated to the underside of the filters were fixed and stained with crystal violet. Representative images of invasive cells are shown. The number of invasive cells was counted. Scale bars are 100 µm. Data are presented as mean ± SD of three independent experiments. ***indicates P < 0.001