Fig. 2
From: miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4
Overexpression of miR-3195 inhibited OS cell proliferation and induced cell apoptosis. A The transfection efficiency of miR-3195 mimic or miR-NC transfected with in U2OS and MG-63 cells was detected by qRT-PCR. B Cell viability was measured (12—96 h) via CCK-8 assays in transfected U2OS and MG-63 cells. C Colony formation ability was measured in transfected U2OS and MG-63 cells by colony formation assay. D The level of apoptosis rate in transfected U2OS and MG-63 cells was detected by flow cytometry. E The expression levels of apoptosis rated protein Bcl-2, Bax and Cleaved-caspase-3 in transfected U2OS and MG-63 cells were detected by Western blotting. Data are presented as mean ± SD of three independent experiments. ***indicates P < 0.001