Table 1 Primers used in quantitative real-time polymerase chain reaction for gene expression analysis
Gene | Primer sequence (5′–3′) |
|---|---|
Collagen II-F | CGCCATGAAAGTCTTCTGCAACA |
Collagen II-R | CACCAGTTCTTCCGAGGCACA |
Aggrecan-F | CGCTGGTCTGATGGACACTC |
Aggrecan-R | AGATCATCACTACGCAGTCCT |
ADAMTS-4-F | GCTGTGATCGAATCATTGGCT |
ADAMTS-4-R | GACCACATCGCTGTATCCGT |
MMP-3-F | TGTCTTTGAAGCATTTGGGTTT |
MMP-3-R | GTTGCTCTTCAATATGTGGGT |
Sema3A-F | AGGACTCACATTTTGAAAACGG |
Sema3A-R | GATAGCAAAGTCTCGTCCCAT |
CD31-F | AGGTGCTATTCTATAAGGACGAT |
CD31-R | TGTTCAGTATCACGGTGCATT |
NF200-F | AGGACCGTCATCAGGTAGACA |
NF200-R | TCCAGGGCCATCTTGACGTTG |
P65-F | GGGTACATCCGATCCATACGTC |
P65-R | ACCTAATTCCGAGTAGGGCAC |
β-actin-F | CTGAACGTGAAATTGTCCGAGA |
β-actin-R | TTGCCAATGGTGATGACCTG |