Fig. 7From: Pure platelet-rich plasma promotes semaphorin-3A expression: a novel insight to ameliorate intervertebral disk degeneration in vitroInhibition of NF-κB pathway exerted a protected effect on IL-1β-induced NPCs and promoted Sema3A activity. A, B Real-time PCR showed that both L-PRP and P-PRP increased the mRNA of Sema3A and dwindled p65 expression. C–F Western blotting analyzed that the increase in p65 phosphorylation/p65 ratio induced by IL-1β was inhibited by PRP. G–I Immunofluorescence staining showed that P-PRP distinctly suppressed NF-κB pathway and increased Sema3A expression (Bar = 100 μm). ap < 0.05 versus control; bp < 0.05 versus NPCs treated with IL-1β only; and cp < 0.05 versus NPCs treated with IL-1β + P-PRPBack to article page