Fig. 6

PRP improved Sema3A activity in rat IVD tissue induced by IL-1β. A–C RT-qPCR was used to detect the effect of L-PRP and P-PRP on the mRNA expression of Sema3A, CD31, and NF200. D–G The protein expression was observed by western blot assay (Bar = 100 μm). H The positive expression was tested by immunohistochemical staining (bar). ^ap < 0.05 versus control; ^bp < 0.05 versus NPCs treated with IL-1β only; ^cp < 0.05 versus NPCs treated with IL-1β + P-PRP