Fig. 5

PRP-ameliorated ECM degradation in IL-1β-induced NPCs. A–D The gene expression of collagen II, aggrecan, MMP-3, and ADAMTS-4 in NPCs was analyzed by Quantitative real-time PCR. E–I The western blot assays were used to detect the protein expression. J–M Immunofluorescence staining was further taken for positive expression (Bar = 100 μm). ^ap < 0.05 versus control; ^bp < 0.05 versus NPCs treated with IL-1β only; and ^cp < 0.05 versus NPCs treated with IL-1β + P-PRP