Fig. 5

Effects of Lox and/or HIF-1α on the ectopic bone formation induced by BMP9 in 3T3-L1 cells A Effect of BMP9, siRNA of Lox, HIF-1α, and/or siRNA of HIF-1α on bone formation. B Effect of BMP9, Lox knockdown, HIF-1α and/or HIF-1α knockdown on bone formation in 3T3-L1 cells (TV: total volume, BV bone volume, BS: bone surface area, Tb.N: trabecular number, Tb.Th: trabecular thickness, Tb.Sp: trabecular separation). C H&E staining show the effect of BMP9, Lox knockdown, HIF-1α and/or HIF-1α knockdown on bone formation in 3T3-L1 cells (Scale bar: 200 μm for upper panel, 50 μm for lower panel. *p < 0.05, **p < 0.01 vs. group treated with BMP9