Fig. 6

microRNA-20a-5p affected the osteogenic effect of carbamazepine by regulating the ubiquitin-specific peptidase 10/S-phase kinase-associated protein 2 axis. A USP10 and SKP2 levels were detected by WB. B ALP activity detection. C ARS staining was used to detect calcium deposits. Scale bar = 100 µm. The expression of Runx2, ALP, Osx, OCN and Collagen I by RT-qPCR (D) and WB (E). The measurement data were presented as mean ± standard error. All data were obtained from at least three replicate experiments. *p < 0.05, ** p < 0.01, ***p < 0.001