Fig. 5

Ubiquitin-specific peptidase 10 increased S-phase kinase-associated protein 2 stability and promoted S-phase kinase-associated protein 2 expression. A WB was used to detect SKP2 levels. B The USP10 and SKP2 levels were detected by WB. C Co-IP was used to verify the combination of USP10 and SKP2. D Cycloheximide chase assay verified the effect of the stability of SKP2 protein. The measurement data were presented as mean ± standard error. All data were obtained from at least three replicate experiments. *p < 0.05, ** p < 0.01, ***p < 0.001