Fig. 4

Knockdown of ubiquitin-specific peptidase 10 blocked the osteogenic inhibition induced by carbamazepine. A The levels of USP10 were detected by RT-qPCR. B USP10 level was detected by RT-qPCR. ALP activity (C) detection and ARS staining (D) were used to check osteogenic differentiation ability. Scale bar = 100 µm. RT-qPCR (E) and WB (F) were used to detect the expression of Runx2, ALP, Osx, OCN and Collagen I. The measurement data were presented as mean ± standard error. All data were obtained from at least three replicate experiments. *p < 0.05, ** p < 0.01, ***p < 0.001