Fig. 2

microRNA-20a-5p alleviated the inhibition of osteogenic differentiation induced by carbamazepine. A–B The miR-20a-5p level was detected by RT-qPCR. C ALP activity detection. D. ARS staining was used to detect calcium deposits. Scale bar = 100 µm. RT-qPCR (E) and WB (F) were used to detect the expression of Runx2, ALP, Osx, OCN and Collagen I. The measurement data were presented as mean ± standard error. All data were obtained from at least three replicate experiments. *p < 0.05, ** p < 0.01, ***p < 0.001