Fig. 6
From: Adipose-derived stem cell exosome NFIC improves diabetic foot ulcers by regulating miR-204-3p/HIPK2
ADSC-exo si-NFIC expedited HG-mediated HUVEC proliferation, migration, and angiogenesis by regulating miR-204-3p. HUVECs were incubated with PBS, ADSC-exo, ADSC-exosi−NC, ADSC-exosi−NFIC, ADSC-exosi−NFIC + miR-NC, or ADSC-exosi−NFIC + miR-204-3p, followed by HG treatment. A RT-qPCR analysis of miR-204-3p expression in treated HUVECs. B Western blot analysis of HIPK2 protein level in HUVECs. C and D CCK-8 and EdU analysis of HUVEC proliferative ability. E Flow cytometry analysis of HUVEC apoptosis rate. F Wound healing analysis of HUVEC migration rate. G Tube formation analysis of HUVEC tube formation ability. *P < 0.05