Fig. 5
From: Adipose-derived stem cell exosome NFIC improves diabetic foot ulcers by regulating miR-204-3p/HIPK2
Transfer of ADSC exosome NFIC to HUEVC promotes the expression of miR-204-3p. A and B The representative micrograph of round-shaped vesicles by TEM. B Concentration and size distribution of exosomes was examined using NTA. C CD81 and CD63 protein levels in ADSC and ADSC-exo were assessed using western blot assay. D Overexpression or knockdown efficiency of NFIC in ADSCs was determined by western blot assay. E NFIC protein level was measured in ADSC-exo with pcDNA, pcDNA-NFIC, si-NC, or si-NFIC using western blot assay. (F–H) HUVECs were incubated with PBS, ADSC-exo, ADSC-exopcDNA, ADSC-exopcDNA−NFIC, ADSC-exosi−NC, or ADSC-exosi−NFIC. F Western blot analysis of NFIC protein level in HUVECs. G miR-204-3p expression was detected using RT-qPCR in HUVECs. H HIPK2 protein level was measured using western blot assay in HUVECs. *P < 0.05