Fig. 4

NFIC increased miR-204-3p expression. A Binding sites and mutation sites of transcription factor NFIC and miR-204 promoter sequence are shown. B Overexpression efficiency of NFIC was detected using western blot assay. C A dual-luciferase reporter assay was applied to analyze the binding between NFIC and miR-204 promoter. D and E RT-qPCR and western blot assays were employed to measure the expression level of NFIC in DFU tissues and normal tissues. F Western blot analysis of NFIC protein level in HUVECs treated with HG or NG. G Expression correlation between miR-204 and NFIC in DFU patients was analyzed using Pearson correlation analysis. H Effect of NFIC upregulation on miR-204 expression in HUVECs was monitored by RT-qPCR. I Western blot analysis of HIPK2 protein level in HUVECs transfected with pcDNA-NC, pcDNA-NFIC, pcDNA-NFIC + In-miR-NC, and pcDNA-NFIC + In-miR-204-3p. *P < 0.05