Fig. 2
From: Adipose-derived stem cell exosome NFIC improves diabetic foot ulcers by regulating miR-204-3p/HIPK2
HIPK2 is the target gene of miR-204-3p. A Predicated results by TargetScan. B miR-204-3p expression was measured using RT-qPCR in HUVECs transfected with miR-204-3p or miR-NC. C The binding miR-204-3p to HIPK2 in HUVECs was validated by dual-luciferase reporter assay. D RT-qPCR analysis of HIPK2 expression in 27 DFU tissues and 27 normal tissues. E HIPK2 protein level was assessed using western blot assay in DFU tissues and normal tissues. F HIPK2 level was examined using RT-qPCR in HUVECs treated with NG or HG. G Pearson correlation analysis was utilized to evaluate the expression association between miR-204-3p and HIPK2 in DFU tissues. H Knockdown efficiency of miR-204-3p in HUVECs was assessed using RT-qPCR. I HIPK2 protein level was determined in HUVECs transfected with In-miR-NC, In-miR-204-3p, miR-NC, or miR-204-3p using western blot assay. *P < 0.05