Fig. 4

Over-expression of hsa_circ_0088214 suppressed osteosarcoma cells MG63 and U2OS growth, cisplatin resistance but increased apoptotic ratio induced by H₂O₂. A Growth curve of MG63 treated with vector plasmid, circRNA over-expression plasmid, circRNA over-expression plasmid + SC79 or SC79. ^*P < 0.05, ^**P < 0.01 compared to Vector group. B Growth curve of U2OS with above treatments. ^**P < 0.01 compared to Vector group. C Drug resistance assay of MG63 with above treatments under different concentrations of cisplatin. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared to Vector group. D Drug resistance assay of U2OS with above treatments under different concentrations of cisplatin. ^**P < 0.01, ^***P < 0.001 compared to Vector group. E Hoechst 33342 staining of MG63 and U2OS with above treatments induced by 600 μM H₂O₂ for 12 h. F, G Cell cycle analysis of MG63 and U2OS treated with vector plasmid or circRNA over-expression plasmid. H, I Apoptosis analysis of MG63 and U2OS treated with vector plasmid or circRNA over-expression plasmid induced by 600 μM H₂O₂ for 12 h. ^***P < 0.001 compared to Vector group