Fig. 2

The biological function of endothelial cells and expression of miR-214-3p in co-culture system. A β-Galactosidase senescence staining of endothelial cells after co-cultured with normal osteoblasts (Control) or senescent osteoblasts (Model) and GW4869 exosome inhibitor (Model + GW). B Hoechst apoptosis staining of endothelial cells after co-cultured with normal osteoblasts (Control) or senescent osteoblasts (Model) and GW4869 exosome inhibitor (Model + GW). C Ki67 proliferation staining of endothelial cells after co-cultured with normal osteoblasts (Control) or senescent osteoblasts (Model) and GW4869 exosome inhibitor (Model + GW). D Expression of miR-214-3p in senescent osteoblasts (OBs) and senescent vascular endothelial cells (VECs). E Expression of miR-214-3p in vascular endothelial cells after co-cultured with normal osteoblasts (Control) or senescent osteoblasts (Model) and GW4869 exosome inhibitor (Model + GW). (∗ means comparison with the control group, p < 0.05; # means comparison with the model group, p < 0.05; ∗∗ means comparison with the control group, p < 0.01; ## means comparison with the model group, p < 0.01)