Table 1 Primer sequences used in quantitative real-time reverse transcription polymerase chain reaction
Species | Gene target | Sequence |
|---|---|---|
Human | LC3 | Forward: 5′–GATGTCCGACTTATTCGAGAGC–3′ |
Reverse: 5′–TTGAGCTGTAAGCGCCTTCTA–3′ | ||
ATG5 | Forward: 5′–AAAGATGTGCTTCGAGATGTGT–3′ | |
Reverse: 5′–CACTTTGTCAGTTACCAACGTCA–3′ | ||
Beclin1 | Forward: 5′–GGTGTCTCTCGCAGATTCATC–3′ | |
Reverse: 5′–TCAGTCTTCGGCTGAGGTTCT–3′ | ||
PINK1 | Forward: 5′–GGAGCGAGATCCCTCCAAAAT–3′ | |
Reverse: 5′–GGCTGTTGTCATACTTCTCATGG–3′ | ||
GAPDH | Forward: 5′–CTCCCTAACCGTCTCCGCT–3′ | |
Reverse: 5′–GGCTCTCCGCCTGTTTTTC–3′ | ||
Mouse | IL-1β | Forward: 5′–TGCCACCTTTTGACAGTGATG–3′ |
Reverse: 5′–TGATGTGCTGCTGCGAGATT–3′ | ||
IL-6 | Forward: 5′–TGATGGATGCTACCAAACTGG–3′ | |
Reverse: 5′–TTCATGTACTCCAGGTAGCTATGG–3′ | ||
iNOS | Forward: 5′–AATCTTGGAGCGAGTTGTGG–3′ | |
Reverse: 5′–CAGGAAGTAGGTGAGGGCTTG–3′ | ||
Arg1 | Forward: 5′–CAGAAGAATGGAAGAGTCAG–3′ | |
Reverse: 5′–CAGATATGCAGGGAGTCACC–3′ | ||
18sRNA | Forward: 5′–GCAATTATTCCCCATGAACG–3′ | |
Reverse: 5′–GGGACTTAATCAACGCAAGC–3′ |