Fig. 5

Effect of Klotho pretreatment on hPDLSC cellular autophagy activity in vivo and vitro. Scale bar = 20 µm. A Immunofluorescence images of HNA (green fluorescence), LC3 (red fluorescence), and DAPI (blue fluorescence). B RT-qPCR analysis of cellular autophagy gene expression levels in macrophages. C Western blot analysis and quantification of cellular autophagy activity. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant