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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Klotho enhances bone regenerative function of hPDLSCs via modulating immunoregulatory function and cell autophagy

Fig. 4

Klotho-pretreated hPDLSC conditioned medium has an anti-inflammatory effect on Raw264.7 macrophages, in vitro. A CCK8 assay of macrophage viability. B Flow cytometric analysis of macrophage polarization and the percentages of M1 and M2 subtypes. C Western blot analysis and RT-qPCR of macrophage polarization. D RT-qPCR analysis of IL-1β and IL-6 gene expression levels in macrophages. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant. Raw264.7 macrophages were treated according to the groups described in "Flow cytometric assessment of surface markers" section and were divided into 4 groups: Uninduced; Induced (short for M1 induced); Induced + CM-Ctrl (short for hPDLSC-CM-control + M1 induced); Induced + CM-Klotho (short for hPDLSC-CM-Klotho + M1 induced)

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X