Fig. 2

Effect of Klotho pretreatment on hPDLSC bone regenerative ability at the transplantation site. A Micro-CT and quantitative analysis of the new bone regeneration areas in vivo. B HE staining analysis of the new bone growth in the cranial defects. C Masson’s trichrome staining analysis of the new bone growth in the cranial defects. D Expression levels of BSP and TRAP staining in each group, and quantitative data (BSP expression and TRAP-positive cells). Scale bar = 200 µm (B, C) and 100 µm (D). *P < 0.05, **P < 0.01