Fig. 4

CPEB2 regulated ARPC5 expression. A ARPC5 mRNA expression levels in the bone marrow CD138+ plasma cells from 20 MM patients and healthy normal donors was measured by qRT-PCR. B ARPC5 protein expression levels in the bone marrow CD138+ plasma cells from five MM patients (M1–M5) and five healthy normal donors (N1–N5) was measured by western blot. C The correlation between CPEB2 and ARPC5 in MM was analyzed by Pearson method. D Western blot analysis was used to detect ARPC5 protein expression in MM cells and nPCs cells. E FISH assay was used to assess the co-localization of CPEB2 and ARPC5 in OPM2 cells. Scale bar, 50 μM. F, G ARPC5 mRNA and protein expression levels were examined by qRT-PCR and western blot analysis in OPM2 cells transfected with sh-CPEB2 or U266 cells transfected with pc-CPEB2. H Actinomycin D was used to assess the stability of ARPC5 in OPM2 cells transfected with sh-CPEB2 or U266 cells transfected with pc-CPEB2. I CHX treatment was performed to assess the effect of sh-CPEB2 or pc-CPEB2 on the half-life of ARPC5. J RIP assay was used to confirmed the interaction between CPEB2 and ARPC5 in U266 cells. Cell experiment was repeated three times. Data were presented as the mean ± SD and analyzed by Student’s t test (for two groups) or one-way ANOVA followed by Tukey post hoc test (for multiple groups). *P < 0.05, **P < 0.01, ***P < 0.001