Fig. 1From: Aspirin prevents estrogen deficiency-induced bone loss by inhibiting osteoclastogenesis and promoting osteogenesisAspirin inhibited RANKL-induced osteoclastogenesis in a dose-dependent manner without cytotoxicity. A Representative images of TRAP staining treated with increasing concentrations of aspirin. B Quantitative analysis of the number of TRAP-positive cells with more than 3 nuclei. C The cytotoxicity of aspirin was evaluated by CCK-8 assay. D Representative images of fluorescence staining treated with various concentrations of aspirin. E Quantitative analysis of the number of osteoclasts. F Representative images of hydroxyapatite resorption treated with different concentrations of aspirin. G Quantitative analysis of the area of hydroxyapatite resorption. Scale bar = 10 μm. *P < 0.05, **P < 0.01Back to article page