Fig. 4

ANE restrained the activation of NOX4/NF-κB signaling pathway. A–C The protein expression of NOX4, NF-κB and p- NF-κB were assessed by western blot. D The mRNA expression of NOX4 was assessed by RT-PCR. E–G After transfected with or without pcDNA-NC or pcDNA-NOX4, the mRNA and protein expression of NOX4 was detected by RT-PCR and western blot. **p < 0.01 versus control group. ##p < 0.01 versus H₂O₂ treatment group