Fig. 5

SIRT3 is a direct target of miR-494. a Assumed miR-494 target site in the 3′-UTR of the human SIRT3 transcript projected through bioinformatics analyses. b Luciferase activity in HEK 293 cells co-transfected with miR-494 mimic or mimic control and WT or MUT SIRT3 3′-UTR constructs. c mRNA expression of SIRT3 of NPCs treated above. d The SIRT3 protein content of NPCs treated above. All experiments were performed three times in duplicate, and data are shown as average ± SD (n = 3). *P < 0.05 vs. mimic control. #P < 0.05 vs. the suppressor control