Fig. 4

Effects of miR-494 on IL-1β-induced apoptosis and mitochondrial dysfunction in NPCs. NP cells were untreated or treated with IL-1β (10 ng/mL). a mRNA expression of miR-494 of NPCs treated above. b Representative fluorescence images with ISH and quantitative analysis (original magnification ×400, scale bar: 25 μm). c Transfection efficiency of miR-494 mimic and miR-494 inhibitor were measured using qRT-PCR. Data are shown as average ± SD. *P < 0.05 vs. the control. NPCs were untreated, treated with IL-1β (10 ng/mL) alone or with IL-1β (10 ng/mL) and mimic control or miR-494 suppressor, inhibitor control or miR-494 mimic. d The protein content of Bcl-2 and Bax and the ratio of cleaved caspase-3/caspase-3 of NPCs treated above. e Representative WB assay and quantitation of the Cyt-c content in cytoplasmic and mitochondrial extracts. f Representative fluorescence images with TUNEL staining and quantitative analyses of TUNEL-positive cells (scale bar: 50 μm, original magnification ×200). g Representative fluorescence images with Mito-Sox and quantitative analyses of the fluorescence intensity (original magnification × 1000, scale bar: 10 μm). h Representative fluorescence images with Mitotracker Red and quantitative analyses of the fluorescence intensity (original magnification ×1000, scale bar: 10 μm). All experiments were performed three times in duplicate, and data are shown as average ± SD (n = 3). *P < 0.05 vs. the IL-1β + mimic control. #P < 0.05 vs. the IL-1β + inhibitor control