Fig. 4

miR-382 promotes cell proliferation and osteogenesis in MG63 cell. A. qRT-PCR analysis of miR-382 level in MG63 cells after transfection with negative control, miR-382 mimic or miR-382 inhibitor. B Western blotting analysis of SLIT2 expression in MG63 cells after transfection with negative control, miR-382 mimic or miR-382 inhibitor. C Binding sites between miR-382 and SLIT2 gene predicted by TargetScan website (Left). The regulation of miR-382 on SLIT2 gene transcription is verified by dual-luciferase reporter gene assay (Right). D ALP staining, E alizarin red S staining, F qRT-PCR and G western blot analysis of miR-382, SLIT2 and osteogenesis-associated markers in MG63 cells after transfection with negative control, miR-382 mimic or miR-382 inhibitor. Statistical evaluation was performed using one-way ANOVA. Mean ± SEM. nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. Statistical evaluation was performed using two-way ANOVA. Mean ± SEM. *represents P < 0.05 compared with NC group. *P < 0.05, ***P < 0.001; # represents P < 0.05 compared with HG group. ###P < 0.001.