Fig. 3
From: MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritis
HBEGF controls the homeostasis of chondrocyte extracellular matrix. A–C After 24 and 48 h of transfection, HBEGF overexpression and knockdown efficiency at the mRNA level (A-B, n = 3 with 3 technical replicates, Student's t test (t test) was used for comparison between two groups. *p < 0.05) and protein levels (C, n = 3; three different donors) were assessed in transfected chondrocytes. D, E Chondrogenesis-related gene expression at the mRNA level (D, n = 3 with 3 technical replicates; Student's t test was used for comparisons between two groups. *p < 0.05) and protein levels (E, n = 3; three different donors) were assessed in chondrocytes following HBEGF overexpression. F, G Chondrogenesis-related gene expression at the mRNA level (F, n = 3 with 3 technical replicates; Student's t test was used for comparisons between two groups. *p < 0.05) and protein levels (G, n = 3; three different donors) were assessed in chondrocytes following HBEGF knockdown. OE, overexpression; Sh, short hairpin RNA; NC, negative control; ECM, extracellular matrix