Fig. 1

Effect of combination of PIP and DOX on proliferative activity of osteosarcoma cells. A, B Cytotoxicity analysis of PIP and DOX against U2OS and 143B cell. Varying concentrations of PIP combined DOX were observed to be potent in downregulating the cell viability of U2OS and 143B for 24 h and 48 h via the CCK8 assay. C, D Varying concentrations of PIP combined DOX could effectively decrease the cell viability of U2OS and 143B for 24 h and 48 h via the CCK8 assay. E, F CDI of different PIP combined with DOX for 24 h and 48 h in U2OS and 143B cells. G The PIP + DOX group was more efficient in inhibiting cell viability compared to the DOX group for 24 h and 48 h. *P < 0.05, **P < 0.01, ***P < 0.001