Table 1 Primer sequences for RT-qPCR
From: Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
Gene | Forward primer sequence (5'-3’) | Reverse primer sequence (5'-3’) |
|---|---|---|
Gapdh | ACGGCAAGTTCAACGGCACAG | GAAGACGCCAGTAGACTCCACGAC |
Alpl | CGTTTTCACGTTTGGTGGCT | ACCGTCCACCACCTTGTAAC |
Ibsp | ACAACACTGCGTATGAAACCTATGAC | AGTAATAATCCTGACCCTCGTAGCC |
Runx2 | CAGATTACAGATCCCAGGCAGAC | AGGTGGCAGTGTCATCATCTGAA |
Spp1 | GAGCAGTCCAAGGAGTATAAGC | AACTCGTGGCTCTGATGTTC |
Col1a1 | CGAGTATGGAAGCGAAGGTT | CTTGAGGTTGCCAGTCTGTT |
Lc3 | GCGAGTTGGTCAAGATCATCC | CGTCTTCATCCTTCTCCTGTTC |
Becn1 | AATCTAAGGAGTTGCCGTTGT | GCCTCCAGTGTCTTCAATCTT |
Atg5 | GAAGGCACACCCCTGAAATG | CCTCAACTGCATCCTTGCAC |