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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway

Fig. 4

KIF4A was highly expressed in OS cells and interacted with DEPDC1. A The interaction between DEPDC1 and KIF4A was confirmed by STRING. B/C The expression of KIF4A in U2OS cells was detected by RT-qPCR and western blot. ***P < 0.001 versus hFOB1.19 group. D The expression of KIF4A and DEPDC1 in proteins incubating with anti-DEPDC1 was detected by western blot. E The expression of KIF4A and DEPDC1 in proteins incubating with anti-KIF4A was detected by western blot. ***P < 0.001 versus Input group. ###P < 0.001 versus Input group. F The expression of KIF4A in U2OS cells transfected with sh-DEPDC1 was detected by western blot. ***P < 0.001 versus Control group. G The expression of DEPDC1 in U2OS cells transfected with Ov-KIF4A was detected by western blot

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X