Fig. 6

RhoA/ROCK activator weakened the inhibitory effect of nesfatin-1 in RhoA/ROCK pathway in OA chondrocytes. A The expression of RhoA/ROCK downstream proteins (Cofilin-2, p-Cofilin-2, LIMK1 and p-LIMK1) in OA chondrocytes in different groups determined by western blot and quantified by ImageJ. B The activity of RhoGTPase in OA chondrocytes in different groups determined by RhoGTP ELISA kit. Data are expressed as means ± SD of 3 independent experiments. **p < 0.01 compared with control group; ^#p < 0.05, ^##p < 0.01, compared with IL-1β group; ^§§p < 0.01, ^§§§p < 0.001 compared with IL-1β + NES group